Quantitation of mRNA by Polymerase Chain Reaction
Köhler, Thomas / Laßner, Dirk / Remke, Harald / Thamm, Barbara / Pustowoit, Barbara / Rost, Anne-Katrin
In this laboratory "cook-book", the authors provide a concise guide to PCR-based techniques to quantify nucleic acids in biological and clinical samples using exclusively nonradioactive detection methods, e.g. HPLC, biotin and digoxigenin based protocols. Each method presentation also includes sections on theory, reagents, standards, applicability, limitations, and trouble shooting. In addition to the protocols, the authors also provide the ne...